The changes of the secondary structure of collagen type I can be identified by spectral deconvolution, which potentially can provide an automatic diagnosis for fibrotic tissues in the clinical applicaion.Ĭollagen Extracellular matrix Non-destructive imaging Pathological tissue remodeling Raman microspectroscopy Spectral deconvolution.Ĭopyright © 2023 The Author(s). In this study, we propose a non-destructive technique, Raman microspectroscopy, to discriminate fibrotic changes of collagen type I based on a molecular biomarker. Although, several non-invasive imaging techniques such as positron emission tomography, single-photon emission computed tomography and second harmonic generation are gradually employed in preclinical or clinical studies, these techniques are limited in spatial resolution and the morphological interpretation highly relies on individual experience and knowledge. STATEMENT OF SIGNIFICANCE: Current diagnosis of fibrosis still relies on histopathological examination with invasive biopsy procedures. Raman spectroscopy-based methods in combination with this newly discovered spectroscopic biomarker potentially offer a diagnostic approach to non-invasively track and monitor the progression of fibrosis. Statistically significant differences were identified in the amide I region of the spectral subpeak at 1608 cm -1, which was deemed to be representative for structural changes in COL I fibers in all examined fibrotic tissues. Spectral deconvolution of COL I Raman spectra allowed the discrimination of fibrotic and non-fibrotic COL I fibers. In this study, the ECM structures of diseased and healthy human tissue from multiple organs were investigated for the presence of fibrosis using routine histology and marker-independent Raman microspectroscopy and Raman imaging. Increasing fibrosis can lead to organ failure and death therefore, developing methods that potentially allow real-time monitoring of early onset or progression of fibrosis are highly valuable. It is often caused by chronic inflammation, which over time, progressively leads to an excess deposition of collagen type I (COL I) that replaces healthy tissue structures, in many cases leaving a stiff scar. Electronic address: is a consequence of the pathological remodeling of extracellular matrix (ECM) structures in the connective tissue of an organ. 7/1, Eberhard Karls University, 72076 Tübingen, Germany Cluster of Excellence iFIT (EXC 2180) "Image-Guided and Functionally Instructed Tumor Therapies", Eberhard Karls University, Tübingen, Germany NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 7 Institute of Biomedical Engineering, Department for Medical Technologies and Regenerative Medicine, Silcherstr.6 Institute of Biomedical Engineering, Department for Medical Technologies and Regenerative Medicine, Silcherstr.5 Anatomy & Regenerative Medicine Institute, School of Medicine, College of Medicine, Nursing and Health Sciences, National University of Ireland Galway, H91 TK33, Galway, Ireland.Young Drive South, MRL 3645 Los Angeles, CA, USA. 4 Department of Medicine/Cardiology, Cardiovascular Research Laboratories, David Geffen School of Medicine at UCLA, 675 Charles E.3 Institute of Pathology and Neuropathology, University Hospital Tübingen, Tübingen, Germany.7/1, Eberhard Karls University, 72076 Tübingen, Germany. 2 Institute of Biomedical Engineering, Department for Medical Technologies and Regenerative Medicine, Silcherstr.7/1, Eberhard Karls University, 72076 Tübingen, Germany Cluster of Excellence iFIT (EXC 2180) "Image-Guided and Functionally Instructed Tumor Therapies", Eberhard Karls University, Tübingen, Germany. 1 Institute of Biomedical Engineering, Department for Medical Technologies and Regenerative Medicine, Silcherstr.
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